Abstract. Silver nanoparticles were prepared by chemical reduction method. Silver nitrate was taken as metal precursor, chondroitin sulfate as stabilizing agent and glucose as reducing agent. The formation of AgNPc was monitored by UV-VIS absorption spectroscopy. UV-VIS spectroscopy revealed the formation of AgNPc by showing typical surface plasmon absorption maxima at 420-429 nm in the UV-VIS spectrum. Comparison of theoretical (Mie light scattering theory) and experimental data showed that the diameter of AgNPc in colloidal solution is about 20 nm. X-ray diffraction (XRD), FTIR spectroscopy and UV-VIS spectroscopy were used to characterize the obtained nanoparticles. The peaks in the XRD curves are in good agreement with the standard cubic shape values of metallic silver (JCPDS No. 040783) and no peaks of other crystalline impurity phases were detected.

Key words: Nanoparticles, surface plasmon absorption, UV-VIS and FTIR spectroscopy.